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2',3'-Dideoxythymidine-5'-Triphosphate

CAT: 0691-N-4004Size: 100 mMDry Ice: YesHazardous: No
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CAT#:0691-N-4004Size:100 mM
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
2',3'-Dideoxythymidine-5'-Triphosphate (ddTTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3' end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP) . This technique is valuable for the detection of rare mutations. "I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results." Michal Legiewicz Research Scientist Yale University
Applications
Aptamers
Concentration
100 mM
Purity
≥95% by AX-HPLC
Form
Li+
Buffer
H2O
Molecular Formula
C10H17N2O13P3 (free acid)
Molecular Weight
466.10 g/mole (free acid)
Shipping Conditions
Frozen
Storage Conditions
-20°C or below
Applications Notes
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.
Product Name Synonym
5'-Triphosphate

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