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NOTCH1 Protein (C-His, Mouse)

CAT: 1543-P527111Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1543-P527111Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
NOTCH1
Synonyms
9930111A19Rik, lin-12, Mis6, Motch, N1, Notch1, Tan1
Host
Baculovirus-insect Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 82 % as determined by SDS-PAGE
Bioactivity
1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 (cat: 10171-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Immobilized mouse DLL4-his (Cat:50640-M08H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL.
Function
Met1-Gln526
Molecular Weight
The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
Species
Mouse

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