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CD4 Protein (C-His, Human)

CAT: 1543-P520033Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1543-P520033Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
CD4
Synonyms
CD4mut, IMD79, Leu-3, OKT4D, T4
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Bioactivity
1.Flow cytometric analysis of anti-CD4 CAR expression. 5e5 of anti-CD4 CAR-293 cells were stained with 100 μL of 5 μg/mL of AF 647-conjugated human CD4 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10400-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 647 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of AF 647-conjugated CD4 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human CD4 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10400-H86H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) . 3.1E5 of mouse Anti-CD4 antibody-conjugated beads (6-8 μm) were stained with a series of concentrations of AF 647-conjugated human CD4 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10400-H86H-SG) . Binding activity was quantified based on AF 647 fluorescence. The recommended working concentration is 2 μg/mL (end-user titration is recommended for optimal performance) .
Function
Met1-Trp390
Molecular Weight
The protein has a predicted molecular mass of 44 kDa.
Species
Human

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