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Human MDH2 Recombinant Protein

CAT: 0519-PROTP40926-50ugSize: 50 µgDry Ice: NoHazardous: No
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CAT#:0519-PROTP40926-50ugSize:50 µg
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Description
Human MDH2 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 25-338aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Synonyms
Malate dehydrogenase 2 NAD (mitochondrial), M-MDH, MDH, MOR1
Gene Name
MDH2
UniProt
P40926
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Signal Transduction
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 700 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
35.2 kDa (335aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

UniProtKB · P40926

Malate dehydrogenase, mitochondrial

MDHM_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P40926
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MDH2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
1.1.1.37
Processing
Precursor
Secondary accessions
A8K414, B2RE78, B4DE44, E9PDB2, O43682
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationGlycoproteinLipoproteinPalmitatePhosphoprotein

Coding sequence diversity

Alternative splicing

Disease

Disease variantEpilepsy

Cellular component

Mitochondrion

Ligand

NAD

Molecular function

Oxidoreductase

Domain

Transit peptide

Biological process

Tricarboxylic acid cycle