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Mouse DHFR Recombinant Protein

CAT: 0519-PROTP00375-100ugSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0519-PROTP00375-100ugSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Synonyms
Dihydrofolate reductase, EC 1.5.1.3
Gene Name
Dhfr
UniProt
P00375
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Redox Proteins
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
23.8 kDa (207aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

UniProtKB · P00375

Dihydrofolate reductase

DYR_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P00375
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Dhfr
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
1.5.1.3
Processing
—
Secondary accessions
P70693, Q61485, Q61487, Q61579
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationUbl conjugation

Cellular component

CytoplasmMitochondrionNucleus

Biological process

Methotrexate resistanceOne-carbon metabolism

Ligand

NADP

Molecular function

OxidoreductaseRNA-binding

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