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Anti-SETDB1 Antibody

CAT: 0519-A00881Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0519-A00881Size:100 µL
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Description
Boster Bio Anti-SETDB1 Antibody catalog # A00881. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Synonyms
Histone-lysine N-methyltransferase SETDB1;2.1.1.43; ERG-associated protein with SET domain; ESET; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SETDB1; KIAA0067, KMT1E
Gene Name
SETDB1
UniProt
Q15047
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full length Human SETDB1.
Clonality
Polyclonal
Tissue Specificity
Widely expressed. High expression in testis.
Applications
WB, IHC, IF
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration
1 mg/mL
Form
Liquid
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306) . Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306) . Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306) . The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610) .
Storage Conditions
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
143157 MW
Gene Name Synonym
Histone-lysine N-methyltransferase SETDB1
Subcellular Location
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
Protein Name
Histone-lysine N-methyltransferase SETDB1
Contents
Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

UniProtKB · Q15047

Histone-lysine N-methyltransferase SETDB1

SETB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15047
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SETDB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.1.1.366
Processing
—
Secondary accessions
A6NEW2, Q5SZD8, Q5SZD9, Q5SZE0, Q5SZE7, Q96GM9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Chromatin regulatorMethyltransferaseRepressorTransferase

Cellular component

ChromosomeCytoplasmNucleus

Domain

Coiled coilRepeat

PTM

Isopeptide bondMethylationPhosphoproteinUbl conjugation

Ligand

Metal-bindingS-adenosyl-L-methionineZinc

Biological process

TranscriptionTranscription regulation

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