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Human NOTCH3 ELISA Kit PicoKine®

CAT: 0519-EK2275Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK2275Size:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Neurogenic locus notch homolog protein 3 (Notch 3) is a protein that in humans is encoded by the NOTCH3 gene. This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) .
Description
Human NOTCH3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human NOTCH3 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 25 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 60 85 52 67 2.73 4.1% Sample 2 171 126 181 177 164 8.7 5.3% Sample 3 1100 1319 1144 911 1119 83.22 7.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 60 85 52 67 2.73 4.1% Sample 1 70 60 85 52 67 2.73 4.1% Sample 2 171 126 181 177 164 8.7 5.3% Sample 2 171 126 181 177 164 8.7 5.3% Sample 3 1100 1319 1144 911 1119 83.22 7.4% Sample 3 1100 1319 1144 911 1119 83.22 7.4%
Gene Name
NOTCH3
Gene ID
4854
UniProt
Q9UM47
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: A40-E467
Capture Antibody
Polyclonal antibody from goat
Detection Antibody
Polyclonal antibody from goat
Applications
ELISA
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA) .
Detection Range
46.9 pg/mL - 3,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 171 1100 65 148 845 Standard deviation 3.89 13.68 84.07 1.37 6.72 47.49 CV (%) 5.6% 8% 7.4% 2.1% 4.5% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 171 1100 65 148 845 Standard deviation 3.89 13.68 84.07 1.37 6.72 47.49 CV (%) 5.6% 8% 7.4% 2.1% 4.5% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 70 171 1100 65 148 845 Mean (pg/ml) 70 171 1100 65 148 845 Standard deviation 3.89 13.68 84.07 1.37 6.72 47.49 Standard deviation 3.89 13.68 84.07 1.37 6.72 47.49 CV (%) 5.6% 8% 7.4% 2.1% 4.5% 5.6% CV (%) 5.6% 8% 7.4% 2.1% 4.5% 5.6%
Sensitivity
<25 pg/mL
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity
Natural and recombinant human NOTCH3
Anticoagulant
Heparin or EDTA
Sample Data
Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

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