MDM2 Polyclonal Antibody

• Background :

  MDM2 encodes a nuclear-localized E3 ubiquitin ligase. The MDM2 proto-oncogene can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

• Description :

  Rabbit Anti-MDM2 Polyclonal Antibody

• Product Name Alternative :

  MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2

• Gene ID :

  4193

• Accession Number :

  Q00987

• Reactivity :

  Human, Mouse

• Immunogen :

  Synthesized peptide derived from the C-terminal region of human MDM2

• Clonality :

  Polyclonal

• Tag :

  E3 ubiquitin-protein ligase Mdm2

• Applications :

  IF, WB, IHC, ELISA

• Purification Method :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration :

  1 mg/ml

• Molecular Weight :

  55kD

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes :

  MDM2 Polyclonal Antibody detects endogenous levels of MDM2 protein.

• Formulation :

  Liquid solution

• Buffer pH :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes :

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200; WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; Not yet tested in other applications

• Other Product Names :

  MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2

• Tested Applications :

  WB, IHC, IF, IP, ELISA

• Host or Source :

  Rabbit

• Accession Number URL :

  http://www.uniprot.org/uniprot/Q00987

• Isotype :

  Rabbit IgG