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Taq DNA Ligase

CAT: 1465-EG20201SSize: 1000 UDry Ice: NoHazardous: No
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CAT#:1465-EG20201SSize:1000 U
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl ends of adjacent oligonucleotide chains that hybridize to the same complementary target DNA strand. This reaction occurs only when both oligonucleotide chains are fully complementary to the target DNA and there are no gaps between them. Therefore, it can be used for single-base substitution detection, but not the substitute of T4 DNA ligase. Taq DNA Ligase is active at the temperature from 45 to 65°C and requires NAD+ as a coenzyme.
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG20201.pdf
Concentration
40 U/μL
Components
• Taq DNA Ligase (40 U/μl) : 25 μl • 10x Taq DNA Ligase Buffer: 1 ml
Shipping Conditions
2~8°C
Storage Conditions
-20°C
Shelf Life
2 Years

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