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FITC (solution)

CAT: 0804-HY-DY1007-01Size: 1 mLDry Ice: NoHazardous: No
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CAT#:0804-HY-DY1007-01Size:1 mL
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Description
FITC (Fluorescein Isothiocyanate) (solution), is one of the green fluorescein derivatives widely used in biology. FITC has the characteristics of high absorptivity and excellent fluorescence quantum yield. The isothiocyanate group of FITC can be combined with amino, sulfhydryl, imidazole, tyrosyl, carbonyl and other groups on the protein, so as to achieve protein labeling including antibodies and lectins. In addition to its use as a protein marker, FITC can also be used as a fluorescent protein tracer to rapidly identify pathogens by labeling antibodies, or for microsequencing of proteins and peptides (HPLC) . The maximum excitation wavelength of FITC is 494 nm. Once excited, it fluoresces yellow-green at a maximum emission wavelength of 520 nm. In addition, FITC is also a hapten that can induce contact hypersensitivity (CHS) and induce an atopic dermatitis model[1][2][3][4].Solvent and concentration: DMSO: 20 mM
CAS Number
3326-32-7
Target
Fluorescent Dye
Related Pathways
Others
Field of Research
Inflammation/Immunology
Smiles
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(N=C=S)C=C5
Molecular Formula
C21H11NO5S
Molecular Weight
389.38
References & Citations
[1]Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12 (5) :3488-3494. Epub 2016 Oct 5.|[2]Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71|[3]Shigeno T, et al. Phthalate ester‐induced thymic stromal lymphopoietin mediates allergic dermatitis in mice[J]. Immunology, 2009, 128 (1pt2) : e849-e857.
Shipping Conditions
Room temperature
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

Fluorescein 5-isothiocyanate

Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

CAS Number3326-32-7
PubChem CID18730
IUPAC Name3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one
Molecular FormulaC21H11NO5S
Molecular Weight389.4
XLogP4.8
Topological Polar Surface Area120
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count7
Rotatable Bond Count1
Heavy Atom Count28
Formal Charge0
Complexity668
SMILES
C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
InChI
InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChIKey
MHMNJMPURVTYEJ-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Fluorescein 5-isothiocyanate
CAS: 3326-32-7
CID: 18730
Formula: C21H11NO5S
MW: 389.4
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight389.4
XLogP34.8
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count7
Rotatable Bond Count1
Exact Mass389.03579362
Monoisotopic Mass389.03579362
Topological Polar Surface Area120 Ų
Heavy Atom Count28
Formal Charge0
Complexity668
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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