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Blue Tetrazolium

CAT: 0804-HY-D0007-01Size: 100 mgDry Ice: NoHazardous: No
CAT#:0804-HY-D0007-01Size:100 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Blue Tetrazolium (Tetrazolium blue), as a blue dye for microbial research, can be reduced into blue tetrazolium formazan (BTF) . Blue Tetrazolium can be used to determine the activity of succinate dehydrogenase (SDH) in yeast strains, which has been reduced as a substrate. Use DMSO to extract BTF from cells, and test absorption spectrum of BTF. The BTF shows a wide wavelength range of 480-600 nm with maximal absorbance seen at 540 nm. Blue Tetrazolium, combines with succinate dehydrogenase (SDH) [1].
CAS Number
[1871-22-3]
Product Name Alternative
Tetrazolium blue
UNSPSC
12352200
Hazard Statement
H350
Target
Fluorescent Dye
Related Pathways
Others
Field of Research
Others
Solubility
10 mM in DMSO
Smiles
COC(C=C(C1=CC(OC)=C(N2[N+](C3=CC=CC=C3)=NC(C4=CC=CC=C4)=N2)C=C1)C=C5)=C5N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-]
Molecular Formula
C40H32Cl2N8O2
Molecular Weight
727.64
Precautions
H350
References & Citations
[1]Kregiel D, et al. Succinate dehydrogenase activity assay in situ with blue tetrazolium salt in crabtree-positive Saccharomyces cerevisiae strain[J]. Food Technology and Biotechnology, 2008, 46 (4) : 376-380.
Shipping Conditions
Room Temperature
Storage Conditions
RT, sealed storage, away from moisture and light
Scientific Category
Biochemical Assay Reagents
Clinical Information
No Development Reported

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