Anti-Chromogranin B Antibody

• Description:

  Rabbit Polyclonal Antibody specific to Chromogranin B

• Product Name Alternative:

  Chromogranin-B, CgB, Secretogranin I, SgI [Cleaved into: PE-11; GAWK peptide; CCB peptide]

• Gene Name:

  CHGB

• NCBI Gene ID:

  CHGB

• UniProt:

  P05060

• Cellular Locus:

  Secreted. Note=Neuroendocrine and endocrine secretory granules.

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide derived from the C-terminus (aa 641-655) of chromogranin B (accession no. NP_001810. This sequence is identical in human, mouse, and rat chromogranin B.

• Target Antigen:

  Secretogranin-1

• Target:

  Chromogranin B

• Clonality:

  Polyclonal

• Type:

  Antibody

• Applications:

  WB, IHC

• Field of Research:

  Hormones/Proteins

• Purification Method:

  Whole antiserum

• Concentration:

  Lot Specific

• Dilution:

  Dilute in PBS or medium that is identical to that used in the assay system.

• Format:

  Whole Antiserum

• Form:

  Liquid

• Buffer:

  Whole Antiserum

• Function:

  Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.

• Additionnal Information:

  Immunoblotting: use diluted 1:2,500- 1:10,000. A band of 110kDa is detected. Although the predicted mw of CgB is ~76kDa, the highly acidic nature of CgB causes it to run at a higher mw in SDS- PAGE.
  
  Immunohistochemistry: use diluted 1:500- 1:5,000.

• Storage Conditions:

  This antibody is stable for at least one (1) year at -20°C to -70°C. Avoid multiple freeze-thaw cycles.

• Specificity:

  This antibody recognizes human, mouse, and rat chromogranin B.

• Formulation:

  Whole antiserum

• Target Background:

  Chromogranin B (CgB, secretogranin I) is a secretory granule matrix protein expressed in a wide variety of endocrine cells and neurons where it may have both extracellular and intracellular functions. Extracellular roles for CgB are dependent on its proteolytic processing within secretory granules to form smaller peptides that may have a role in the neuroendocrine/sympathoadrenal stress response to systemic infection, and CgB fragments may also have an autocrine inhibitory role in the release of co-stored hormones, such as insulin.

• CAS Number:

  9007-83-4