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Mouse EGF protein

CAT: 0749-PRP1957-01Size: 10 mgDry Ice: NoHazardous: No
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CAT#:0749-PRP1957-01Size:10 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.EGF Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.
Description
MEGF
Product Name Alternative
Beta-urogastrone, EGF, epidermal growth factor, MEGF, HOMG4, URG, Urogastrone
Gene ID
13645
Tag
MEGF
Applications
No tag
Purity
> 95% as determined by SDS-PAGE.
Activity
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.017 ng/mL. The specific activity of recombinant Mouse EGF is approximately >5.89 x 107IU/mg
Molecular Formula
The protein has a calculated MW of 6.2 kDa.
Molecular Weight
The protein has a calculated MW of 6.98 kDa.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles
Protein Length
The recombinant mouse EGF consists of 55 amino acids and predicts a molecular mass of 6.2 KDa
Formulation
The protein was lyophilized from sterile 150 mM NaCl 20 mM Tris , pH 8.0. If you have any concerns or special requirements, please confirm with us.
Applications Notes
Mouse
Other Product Names
EGF, beta-urogastrone, EGF, epidermal growth factor, MEGF, HOMG4, URG, Urogastrone
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse EGF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Preparation
E. coli
AA Sequence
Amino acid sequence derived from Mouse EGF (Met978-Arg 1029) (P01132) was expressed

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