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Monoclonal Anti-E2b Tag

CAT: 1510-BTL1004Size: 100 μgDry Ice: NoHazardous: No
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CAT#:1510-BTL1004Size:100 μg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
E2 Tags are 10-amino acids (epitope E2a: SSTSSDFRDR) - and 12 amino acids (epitope E2b: GVSSTSSDFRDR) -long peptides derived from the E2 protein of bovine papillomavirus type 1. The modules for C-terminal tagging with E2a and E2b epitopes were constructed by the modification of the pYM-series plasmid. The N-terminal E2a and E2b tagging modules were based on pOM-series plasmid.
Host
Mouse
Reactivity
All
Immunogen
A synthetic peptideGVSSTSSDFRDRconjugated to KLH.
Applications
Western Blot (1:1000-10000)
Form
Liquid
Buffer
In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
References & Citations
1. Tiina Tamm. Plasmids with E2 epitope tags: tagging modules for N- and C-terminal PCR-based gene targeting in both budding and fission yeast, and inducible expression vectors for fission yeast. Yeast Volume 26, Issue 1, pages 55–66, January 2009.
Storage Conditions
Store at -20°C for 1 year. Or aliquot and store at -80°C for long term. Avoid repeated freeze and thaw cycles.
Specificity
Recognize C-terminal, internal and N-terminal E2b tagged proteins.
Isotype
IgG1

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