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Recombinant Human CCL2 Protein

CAT: 1510-BRP1173-1Size: 10 µgDry Ice: NoHazardous: No
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CAT#:1510-BRP1173-1Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemoattractant protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 tightly regulates cellular mechanicsand thereby recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection.
Description
Recombinant human CCL2 is produced by the E.coli system, with the target gene encoding Gln24-Thr99 expressed.
Synonyms
CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF, C-C motif chemokine ligand 2
Source
E.coli
Applications
SDS-PAGE
Endotoxin
<1 EU/µg by LAL test
Purity
>95%by SDS-PAGE
Buffer
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH6.0.
Reconstitution
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)
References & Citations
Evers TM, Sheikhhassani V, Haks MC, Storm C, Ottenhoff TH, Mashaghi A (January 2022) . "Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics". iScience. 25 (1) 103555. Carr MW, Roth SJ, Luther E, Rose SS, Springer TA (April 1994) . "Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant". Proceedings of the National Academy of Sciences of the United States of America. 91 (9) : 3652–6. Xu LL, Warren MK, Rose WL, Gong W, Wang JM (September 1996) . "Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro". Journal of Leukocyte Biology. 60 (3) : 365–71.
Storage Conditions
Shipat 4°C. Upon receipt, aliquot and store at -20°Cor -80°C for long term. Avoid repeated freeze and thaw cycles.
Formulation
Lyophilized
Uniprot Accession Number
P13500

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