Recombinant human COMT protein

• Description :

  Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be located in the postsynaptic neuron and plays roles in the metabolism of catechol estrogens and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT inhibitors prevent levodopa degradation, increase its availability and are used in the treatment of patients with Parkinson's disease. Recombinant human COMT protein was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative :

  Catechol-O-methyltransferase, COMT

• Expression System :

  E.coli

• Antigen Species :

  Human

• Tag :

  Non-Tagged

• Applications :

  SDS-PAGE

• Concentration :

  1 mg/mL (determined by Bradford assay)

• Endotoxin :

  < 1 EU per 1ug of protein (determined by LAL method)

• Purity :

  > 95% by SDS-PAGE

• Molecular Weight :

  24.4 kDa (221aa) confirmed by MALDI-TOF

• Additionnal Information :

  COMT, Catechol-O-methyltransferase, Catechol-O-methyltransferase, Catechol-O-methyltransferase, COMT, COM0905-10 µg, COM0905-20 µg, COM0905-50 µg, COM0905-100 µg, COM0905-250 µg, COM0905-500 µg, COM0905-1 mg, COM0905-10, COM0905-20, COM0905-50, COM0905-100, COM0905-250, COM0905-500, COM0905-1

• References & Citations :

  Cotton NJ., et al. (2004) J Biol Chem. 279 (22) :23710-8. ; ; Dawling S., et al. (2001) Cancer Res. 61 (18) :6716-22.;

• Other References :

  Ishida Y, et al. Yokukansan, a Traditional Japanese Medicine, Enhances the L-DOPA-Induced Rotational Response in 6-Hydroxydopamine-Lesioned Rats: Possible Inhibition of COMT. (Biol Pharm Bull. 2016) {https://pubmed.ncbi.nlm.nih.gov/29707715/}; ; Ikeda M, et al. Inhibitory Effect of Bovine Lactoferrin on Catechol-O-Methyltransferase. (Molecules. 2017) {https://pubmed.ncbi.nlm.nih.gov/28825621/}; ; Ikeda M, et al. Effects of bovine lactoferrin on l-DOPA absorption and metabolism in mice. (Food Funct. 2018) {https://pubmed.ncbi.nlm.nih.gov/26725433/}; ; Nagai M, et al. Comparative examination of levodopa pharmacokinetics during simultaneous administration with lactoferrin in healthy subjects and the relationship between lipids and COMT inhibitory activity in vitro. (Nutr Neurosci. 2020) {https://pubmed.ncbi.nlm.nih.gov/32441567/}

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM MgCl2, 10% glycerol

• Scientific Category :

  Enzymes & Proteases

• NCBI Accession Number :

  NP_000745.1

• Uniprot Accession Number :

  P21964

• Species :

  Human

• AA Sequence :

  MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P