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Recombinant mouse EphA3 protein

CAT: 0112-ATGP4148-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP4148-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EphA3 is a protein-tyrosine kinase that belongs to the ephrin receptor subfamily. It has an extracellular region with a Cys-rich domain and two fibronectin type III repeats, and a single kinase domain. EPH and other EPH-related receptors are involved in various developmental processes, especially in the nervous system. EphA3 interacts with EFNB2 and EFNA5, and is expressed in the forebrain, retinal axons, some motor neurons in the spinal cord, and the heart during development. It regulates axonal guidance and organ formation. It is also found on some blood and solid tumor cells, and on astrocytes near injured nerve tissue. Recombinant mouse EphA3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Specifications
Mouse EphA3 (CAT# ATGP4148) is coated at 2 µg/mL (100 ul/well) can bind Human EFNA5 (CAT# ATGP4147) in a Functional ELISA assay.
Product Name Alternative
Ephrin type-A receptor 3, ephrin type-A receptor 3 isoform1, receptor protein-tyrosine kinase, EPH-like kinase 4, tyrosine-protein kinase TYRO4, tyrosine-protein kinase receptor ETK1, Eph receptor A3, AW492086, Cek4, EK4, End3, ETK1, Hek, Hek4, Mek4, Tyro4
Expression System
HEK293
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human EFNA5 (CAT# ATGP4147) .
Molecular Weight
59.5kDa (527aa)
Additionnal Information
ATGP4148-10 µg, ATGP4148-20 µg, ATGP4148-50 µg, ATGP4148-100 µg, ATGP4148-250 µg, ATGP4148-500 µg, ATGP4148-1 mg, ATGP4148-010, ATGP4148-020, ATGP4148-050, ATGP4148-100, ATGP4148-250, ATGP4148-500, ATGP4148-01M
References & Citations
Kudo, C. et al. (2005) J. Comp. Neurol. 487:255-269.; ; Kilpatrick, T.J. et al. (1996) Mol. Cell. Neurosci. 7:62-74.; ; Stephen, L.J. et al. (2007) Dev. Biol. 302:66-79.; ; Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194-200.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Angiogenesis, Cardiovascular
NCBI Accession Number
NP_034270.1
Uniprot Accession Number
Q8BRB1
Species
Mouse
N Terminal Sequence
Glu 21

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