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Recombinant human CD47 protein

CAT: 0112-ATGP4015-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP4015-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD47, also known as leukocyte surface antigen CD47 isoform 1, has a role in both cell adhesions by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Recombinant human CD47, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Specifications
Human CD47 is coated at 1 µg/mL (100 ul/well) can bind Human SIRP gamma/CD172g (CAT# ATGP4030) in a Functional ELISA assay.
Product Name Alternative
CD47, OA3, IAP, MER6, Leukocyte surface antigen CD47 isoform 1, antigenic surface determinant protein OA3, Integrin-associated protein
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human SIRP gamma/CD172g (CAT# ATGP4030)
Molecular Weight
14.7 kDa (129aa)
Additionnal Information
ATGP3188, ATGP4015-10 µg, ATGP4015-20 µg, ATGP4015-50 µg, ATGP4015-100 µg, ATGP4015-250 µg, ATGP4015-500 µg, ATGP4015-1 mg, ATGP4015-10, ATGP4015-20, ATGP4015-50, ATGP4015-100, ATGP4015-250, ATGP4015-500, ATGP4015-1, ATGP4015-10 µg, ATGP4015-20 µg, ATGP4015-50 µg, ATGP4015-100 µg, ATGP4015-250 µg, ATGP4015-500 µg, ATGP4015-1 mg, ATGP4015-10, ATGP4015-20, ATGP4015-50, ATGP4015-100, ATGP4015-250, ATGP4015-500, ATGP4015-1
References & Citations
Iwamoto C et al., (2014) Exp. Hematol. 42 (3) :163-171.; ; Wang Y et al., (2013) Mol. Ther. 21 (10) :1919-1929.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology, Adhesion & Movement
NCBI Accession Number
NP_001768
Uniprot Accession Number
Q08722
Species
Human
N Terminal Sequence
Gln19

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