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Recombinant mouse HPD protein

CAT: 0112-ATGP3760-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3760-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Hpd, also known as 4-hydroxyphenylpyruvate dioxygenase, is a Fe-containing enzyme that catalyzes the second reaction in the catabolism of tyrosine the conversion of 4-hydroxyphenylpyruvate to homogentisate. Existing as a homodimer, Hpd uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. Defects in the gene encoding Hpd are the cause of tyrosinemia type 3 and hawkinsinuria, both of which are inborn errors of metabolism that are associated with a variety of symptoms, including mental retardation and seizures and hair and urine abnormalities. Recombinant mouse Hpd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
4-hydroxyphenylpyruvate dioxygenase, 4HPPD, Fla, Flp, Hppd, Laf, 4-hydroxyphenylpyruvic acid oxidase, HPPDase, F Alloantigen, F protein
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
47.4 kDa (416aa) confirmed by MALDI-TOF
Additionnal Information
Hpd, 4-hydroxyphenylpyruvate dioxygenase, 4HPPD, Fla, Flp, Hppd, Laf, ATGP3760-10 µg, ATGP3760-20 µg, ATGP3760-50 µg, ATGP3760-100 µg, ATGP3760-250 µg, ATGP3760-500 µg, ATGP3760-1 mg, ATGP3760-10, ATGP3760-20, ATGP3760-50, ATGP3760-100, ATGP3760-250, ATGP3760-500, ATGP3760-1
References & Citations
Wada GH., et al. (1975) J Biol Chem J. 250 (17) :6720-6.; ; Endo F., et al. (1997) J Biol Chem. 26; ;272 (39) :24426-32.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_032303
Uniprot Accession Number
P49429
Species
Mouse
AA Sequence
MTTYNNK GPKPERGRFL HFHSVTFWVG NAKQAASFYC NKMGFEPLAY RGLETGSREV VSHVIKQGKI VFVLCSALNP WNKEMGDHLV KHGDGVKDIA FEVEDCDHIV QKARERGAKI VREPWVEQDK FGKVKFAVLQ TYGDTTHTLV EKINYTGRFL PGFEAPTYKD TLLPKLPRCN LEIIDHIVGN QPDQEMQSAS EWYLKNLQFH RFWSVDDTQV HTEYSSLRSI VVTNYEESIK MPINEPAPGR KKSQIQEYVD YNGGAGVQHI ALKTEDIITA IRHLRERGTE FLAAPSSYYK LLRENLKSAK IQVKESMDVL EELHILVDYD EKGYLLQIFT KPMQDRPTLF LEVIQRHNHQ GFGAGNFNSL FKAFEEEQAL RGNLTDLEPN GVRSGM

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