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Recombinant mouse RalA protein

CAT: 0112-ATGP3699-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3699-100Size:100 µg
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Description
Rala, also known as Ras-related protein Ral-A, is a member of the small GTPase superfamily, Ras family of proteins. Rala mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. Rala is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicle trafficking, and endocytosis. Recombinant mouse Rala protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Ras-related protein Ral-A, 3010001O15Rik, AW322615, Ral, Rasl1, V-ral simian leukemia viral oncogene A, RAS like proto-oncogene A
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.7 kDa (227aa) confirmed by MALDI-TOF
Additionnal Information
V-ral simian leukemia viral oncogene A, V ral simian leukemia viral oncogene A, Ras-related protein Ral-A, Rasl1, Rasl 1, Ras related protein Ral A, RAS like proto-oncogene A, RAS like proto oncogene A, Rala, Ral, AW322615, 3010001O15Rik, , ATGP3699-10 µg, ATGP3699-20 µg, ATGP3699-50 µg, ATGP3699-100 µg, ATGP3699-250 µg, ATGP3699-500 µg, ATGP3699-1 mg, ATGP3699-010, ATGP3699-020, ATGP3699-050, ATGP3699-100, ATGP3699-250, ATGP3699-500, ATGP3699-01M
References & Citations
Cascone I., et al. (2008) EMBO J. 27 (18) :2375-2387.; ; Balasubramanian N., et al. (2010) Curr. Biol. 20 (1) :75-79.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 30% glycerol, 0.1M NaCl, 0.1mM PMSF
Scientific Category
Signal Transduction
NCBI Accession Number
NP_062364
Uniprot Accession Number
P63321
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAANKP KGQNSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLCVFSIT EMESFAATAD FREQILRVKE DENVPFLLVG NKSDLEDKRQ VSVEEAKNRA DQWNVNYVET SAKTRANVDK VFFDLMREIR ARKMEDSKEK NGKKKRKSLA KRIRERC