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Human RalA/RalB antibody

CAT: 0112-ATGA0590-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0590-100Size:100 µL
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Description
RalA and RalB belong to subfamily of Ras-related GTPases. This protein mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. It is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicletrafficking, and endocytosis. RalA and RalB share 85% identity.
Product Name Alternative
Ras-related protein Ral-A, RAL, Ras-related protein Ral-B
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human RALA (1-203aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT41E2
Conjugation
Unconjugated
Applications
ELISA, WB, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
RalB, Ras-related protein Ral-B, Ras related protein RalB, Ras related protein Ral B, RalA, Ras-related protein Ral-A, Ras related protein RalA, Ras related protein Ral A, ATGA0590-10 µg, ATGA0590-20 µg, ATGA0590-50 µg, ATGA0590-100 µg, ATGA0590-250 µg, ATGA0590-500 µg, ATGA0590-1 mg, ATGA0590-010, ATGA0590-020, ATGA0590-050, ATGA0590-100, ATGA0590-250, ATGA0590-500, ATGA0590-01M
References & Citations
Balasubramanian N., et al. (2009) Curr. Biol. 20:75-79.; ; Hunt MC., et al. (2005) J Lipid Res. 46, 2029-32.; ; Wildey G.M., et al. (1993), Biochem. Biophys. Res. Comm., 194: 552-559.; ; Jullien-Flores V., et al. (1995) . J. Biol. Chem. 270: 22473-22477.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenetics and Nuclear Signaling
NCBI Accession Number
NP_005393
Uniprot Accession Number
P11233
WB Description
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human RalA protein; ; Lane 2.: Recombinant human RalB protein; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: NIH3T3 cell lysate; ; Lane 4.: PC3 cell lysate; ; Lane 5.: U87MG cell lysate; ; {ATGA0590-WB2.jpg}
FACS Description
Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained with ATGA0590 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

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