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Recombinant human HPRT protein

CAT: 0112-ATGP3466-004Size: 4 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3466-004Size:4 µg
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Description
Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
Product Name Alternative
Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 15unit/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
Molecular Weight
26.7 kDa (238aa) confirmed by MALDI-TOF
Additionnal Information
HPRT, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, ATGP3466-10 µg, ATGP3466-20 µg, ATGP3466-50 µg, ATGP3466-100 µg, ATGP3466-250 µg, ATGP3466-500 µg, ATGP3466-1 mg, ATGP3466-10, ATGP3466-20, ATGP3466-50, ATGP3466-100, ATGP3466-250, ATGP3466-500, ATGP3466-1
References & Citations
Hladnik U., et al. (2008) Arch Neurol. 65 (9) :1240-3.; ; Sculley DG., et al. (1992) Hum Genet. 90 (3) :195-207191.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 20% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000185
Uniprot Accession Number
P00492
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA

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