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Human HPRT antibody

CAT: 0112-ATGA0496-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0496-050Size:50 µL
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Description
HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.
Product Name Alternative
Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human HPRT (1-218aa) purified from E. coli.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT1D9
Conjugation
Unconjugated
Applications
ELISA, WB, FACS, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
HPRT, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1., ATGA0496-10 µg, ATGA0496-20 µg, ATGA0496-50 µg, ATGA0496-100 µg, ATGA0496-250 µg, ATGA0496-500 µg, ATGA0496-1 mg, ATGA0496-10, ATGA0496-20, ATGA0496-50, ATGA0496-100, ATGA0496-250, ATGA0496-500, ATGA0496-1
References & Citations
Sculley DG, et al. (1993) . Hum. Genet. 90 (3) : 195-207.; ; Stout JT, Caskey CT (1986) . Annu. Rev. Genet. 19: 127-48.; ; Davidson BL, et al. (1991) . Am. J. Hum. Genet. 48 (5) : 951-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000185
Uniprot Accession Number
P00492
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: MCF7 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: A549 cell lysate
FACS Description
Flow cytometry analysis of HPRT in A549 cells. The cell was stained with ATGA0496 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of HPRT in HaLe cells. The cell was stained with ATGA0496 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0496-IF1b.jpg}

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