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Recombinant human FBP2 protein

CAT: 0112-ATGP3235-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3235-500Size:500 µg
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Description
FBP2 belongs to the FBPase class 1 family. The protein is a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Recombinant human FBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 1,500 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.
Product Name Alternative
Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 1,500pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.
Molecular Weight
39.0 kDa (362aa) confirmed by MALDI-TOF
Additionnal Information
FBP2, Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2, ATGP3235-10 µg, ATGP3235-20 µg, ATGP3235-50 µg, ATGP3235-100 µg, ATGP3235-250 µg, ATGP3235-500 µg, ATGP3235-1 mg, ATGP3235-10, ATGP3235-20, ATGP3235-50, ATGP3235-100, ATGP3235-250, ATGP3235-500, ATGP3235-1
References & Citations
Gizak A, Maciaszczyk E, et al. (2008) . Proteins. 72 (1) :209-16.; ; Rakus D, Maciaszczyk E, et al. (2005) . FEBS Lett. 579 (25) :5577-81.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_003828
Uniprot Accession Number
O00757
Species
Human
AA Sequence
MTDRSPF ETDMLTLTRY VMEKGRQAKG TGELTQLLNS MLTAIKAISS AVRKAGLAHL YGIAGSVNVT GDEVKKLDVL SNSLVINMVQ SSYSTCVLVS EENKDAIITA KEKRGKYVVC FDPLDGSSNI DCLASIGTIF AIYRKTSEDE PSEKDALQCG RNIVAAGYAL YGSATLVALS TGQGVDLFML DPALGEFVLV EKDVKIKKKG KIYSLNEGYA KYFDAATTEY VQKKKFPEDG SAPYGARYVG SMVADVHRTL VYGGIFLYPA NQKSPKGKLR LLYECNPVAY IIEQAGGLAT TGTQPVLDVK PEAIHQRVPL ILGSPEDVQE YLTCVQKNQA GS

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