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Recombinant mouse TNF-alpha protein

CAT: 0112-ATGP3184-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3184-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TNF, also known as tumor necrosis factor isoform 1, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. This protein is produced by a wide variety of immune and epithelial cell types. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. Recombinant Mouse TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Specifications
Mouse TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
Product Name Alternative
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.02 ng/mL.
Molecular Weight
18kDa (162aa)
Additionnal Information
TNF-a, Tnf, DIF, TNF-a, TNF-alpha, Tnfa, TNFalpha, Tnfsf1a, TNFSF2, ATGP3184-10 µg, ATGP3184-20 µg, ATGP3184-50 µg, ATGP3184-100 µg, ATGP3184-250 µg, ATGP3184-500 µg, ATGP3184-1 mg, ATGP3184-10, ATGP3184-20, ATGP3184-50, ATGP3184-100, ATGP3184-250, ATGP3184-500, ATGP3184-1, ATGP3184-10 µg, ATGP3184-20 µg, ATGP3184-50 µg, ATGP3184-100 µg, ATGP3184-250 µg, ATGP3184-500 µg, ATGP3184-1 mg, ATGP3184-10, ATGP3184-20, ATGP3184-50, ATGP3184-100, ATGP3184-250, ATGP3184-500, ATGP3184-1
References & Citations
Marafini I., et al. (2015) PLoS One. 10 (5) :e0126291.; ; Zelova H., et al. (2013) Inflamm Res. 62 (7) :641-651.
Other References
Kim MJ, et al. Paeonia lactiflora Root Extract and Its Components Reduce Biomarkers of Early Atherosclerosis via Anti-Inflammatory and Antioxidant Effects In Vitro and In Vivo. (Antioxidants (Basel) . 2021) {https://pubmed.ncbi.nlm.nih.gov/34679642/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_038721
Uniprot Accession Number
P06804
Species
Mouse

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