Recombinant human TNF-alpha protein

Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17. 5 kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.

Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

E.coli

Human

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/mL.

17.5 kDa (158aa) confirmed by MALDI-TOF

TNF-a, Tumor necrosis factor alpha, TNFA, TNFSF2, DIF (differentiation inducing factor), CACHECTIN, DIF, TNF-alpha, Tumor necrosis factor alpha, Tumor necrosis factor alpha, TNF, tumor necrosis factor (TNF superfamily, member 2), TNF superfamily member 2, TNF0501-10 µg, TNF0501-20 µg, TNF0501-50 µg, TNF0501-100 µg, TNF0501-250 µg, TNF0501-500 µg, TNF0501-1 mg, TNF0501-10, TNF0501-20, TNF0501-50, TNF0501-100, TNF0501-250, TNF0501-500, TNF0501-1

Chicoine M.R., et al. (1997), J. Neurooncol. 35: 249-57; ; Ruddle N.H., et al. (1992), Curr. Opin. Immunol. 4: 327-32; ; Wingfield P., et al. (1987), FEBS Lett. 211:179-184;

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Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Hormones & Cytokines

NP_000585.2

P01375

Human