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Recombinant human PDIA4 protein

CAT: 0112-ATGP3138-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3138-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PDIA4, also known as ERP72, is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. It is involved in the catalysis of protein-S-S-bond rearrangement. PDIA3 and PDIA4 act as proteases, protein disulfide isomerases, phospholipases or a combination of these. Recombinant human PDIA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity > 5 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
Product Name Alternative
Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 72, ERP70, ERP72
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity > 5 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
Molecular Weight
72.9 kDa (646aa) confirmed by MALDI-TOF
Additionnal Information
PDIA4, Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 72, ERP70, ERP72, ATGP3138-10 µg, ATGP3138-20 µg, ATGP3138-50 µg, ATGP3138-100 µg, ATGP3138-250 µg, ATGP3138-500 µg, ATGP3138-1 mg, ATGP3138-10, ATGP3138-20, ATGP3138-50, ATGP3138-100, ATGP3138-250, ATGP3138-500, ATGP3138-1
References & Citations
Thomas M., et al. (2010) Synapse. 64 (8) :579-93.; ; Joo JH., et al. (2007) Cancer Res. 67 (16) :7929-36.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl,1mM DTT and 10% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_004902.1
Uniprot Accession Number
P13667
Species
Human
AA Sequence
VAGAEGPDE DSSNRENAIE DEEEEEEEDD DEEEDDLEVK EENGVLVLND ANFDNFVADK DTVLLEFYAP WCGHCKQFAP EYEKIANILK DKDPPIPVAK IDATSASVLA SRFDVSGYPT IKILKKGQAV DYEGSRTQEE IVAKVREVSQ PDWTPPPEVT LVLTKENFDE VVNDADIILV EFYAPWCGHC KKLAPEYEKA AKELSKRSPP IPLAKVDATA ETDLAKRFDV SGYPTLKIFR KGRPYDYNGP REKYGIVDYM IEQSGPPSKE ILTLKQVQEF LKDGDDVIII GVFKGESDPA YQQYQDAANN LREDYKFHHT FSTEIAKFLK VSQGQLVVMQ PEKFQSKYEP RSHMMDVQGS TQDSAIKDFV LKYALPLVGH RKVSNDAKRY TRRPLVVVYY SVDFSFDYRA ATQFWRSKVL EVAKDFPEYT FAIADEEDYA GEVKDLGLSE SGEDVNAAIL DESGKKFAME PEEFDSDTLR EFVTAFKKGK LKPVIKSQPV PKNNKGPVKV VVGKTFDSIV MDPKKDVLIE FYAPWCGHCK QLEPVYNSLA KKYKGQKGLV IAKMDATAND VPSDRYKVEG FPTIYFAPSG DKKNPVKFEG GDRDLEHLSK FIEEHATKLS RTKEEL

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