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Recombinant human MTHFD2 protein

CAT: 0112-ATGP2972-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2972-100Size:100 µg
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Description
MTHFD2 also known as Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial. MTHFD2 protein that possesses three distinct enzymatic activities, methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formate-tetrahydrofolate ligase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. Recombinant human MTHFD2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase mitochondrial, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NMDMC
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
37.2 kDa (344aa) confirmed by MALDI-TOF
Additionnal Information
MTHFD2, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase mitochondrial, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NMDMC, ATGP2972-10 µg, ATGP2972-20 µg, ATGP2972-50 µg, ATGP2972-100 µg, ATGP2972-250 µg, ATGP2972-500 µg, ATGP2972-1 mg, ATGP2972-10, ATGP2972-20, ATGP2972-50, ATGP2972-100, ATGP2972-250, ATGP2972-500, ATGP2972-1
References & Citations
Watkins D., et al. (2011) Genet. 48 (9) : 590-2.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_006627.2
Uniprot Accession Number
P13995
Species
Human
AA Sequence
LAAVRNE AVVISGRKLA QQIKQEVRQE VEEWVASGNK RPHLSVILVG ENPASHSYVL NKTRAAAVVG INSETIMKPA SISEEELLNL INKLNNDDNV DGLLVQLPLP EHIDERRICN AVSPDKDVDG FHVINVGRMC LDQYSMLPAT PWGVWEIIKR TGIPTLGKNV VVAGRSKNVG MPIAMLLHTD GAHERPGGDA TVTISHRYTP KEQLKKHTIL ADIVISAAGI PNLITADMIK EGAAVIDVGI NRVHDPVTAK PKLVGDVDFE GVRQKAGYIT PVPGGVGPMT VAMLMKNTII AAKKVLRLEE REVLKSKELG VATN

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