Recombinant human eEF-2K/EEF2K protein

CAT: 0112-ATGP2837-020Size: 20 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP2837-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Eukaryotic elongation factor-2 kinase, also known as EEF2K, is threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. Recombinant human EEF2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Eukaryotic translation initiation factor 3 subunit F, EIF3S5, eEF-2 kinase, CaMKIII, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
84.6 kDa (748aa)
Additionnal Information
EEF2K, Eukaryotic elongation factor-2 kinase, eEF-2K, HSu93850, eEF2K, ATGP2837-10 µg, ATGP2837-20 µg, ATGP2837-50 µg, ATGP2837-100 µg, ATGP2837-250 µg, ATGP2837-500 µg, ATGP2837-1 mg, ATGP2837-10, ATGP2837-20, ATGP2837-50, ATGP2837-100, ATGP2837-250, ATGP2837-500, ATGP2837-1
References & Citations
Ryazanov A.G., et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:4884-4889; ; Knebel A., et al. (2001) EMBO J. 20:4360-4369
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_037434.1
Uniprot Accession Number
O00418
Species
Human
AA Sequence
MADEDLI FRLEGVDGGQ SPRAGHDGDS DGDSDDEEGY FICPITDDPS SNQNVNSKVN KYYSNLTKSE RYSSSGSPAN SFHFKEAWKH AIQKAKHMPD PWAEFHLEDI ATERATRHRY NAVTGEWLDD EVLIKMASQP FGRGAMRECF RTKKLSNFLH AQQWKGASNY VAKRYIEPVD RDVYFEDVRL QMEAKLWGEE YNRHKPPKQV DIMQMCIIEL KDRPGKPLFH LEHYIEGKYI KYNSNSGFVR DDNIRLTPQA FSHFTFERSG HQLIVVDIQG VGDLYTDPQI HTETGTDFGD GNLGVRGMAL FFYSHACNRI CESMGLAPFD LSPRERDAVN QNTKLLQSAK TILRGTEEKC GSPRVRTLSG SRPPLLRPLS ENSGDENMSD VTFDSLPSSP SSATPHSQKL DHLHWPVFSD LDNMASRDHD HLDNHRESEN SGDSGYPSEK RGELDDPEPR EHGHSYSNRK YESDEDSLGS SGRVCVEKWN LLNSSRLHLP RASAVALEVQ RLNALDLEKK IGKSILGKVH LAMVRYHEGG RFCEKGEEWD QESAVFHLEH AANLGELEAI VGLGLMYSQL PHHILADVSL KETEENKTKG FDYLLKAAEA GDRQSMILVA RAFDSGQNLS PDRCQDWLEA LHWYNTALEM TDCDEGGEYD GMQDEPRYMM LAREAEMLFT GGYGLEKDPQ RSGDLYTQAA EAAMEAMKGR LANQYYQKAE EAWAQMEE

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