Recombinant human EEF2 protein

• Description:

  EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. Recombinant human EEF2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

• Product Name Alternative:

  Elongation factor 2, EEF-2, EF-2, EF2, Eukaryotic translation elongation factor 2, Polypeptidyl-tRNA translocase

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE, Denatured

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Purity:

  > 90% by SDS-PAGE

• Molecular Weight:

  34.3 kDa (308aa)

• Additionnal Information:

  EEF2, elongation factor 2, EEF-2, EF-2, EF2, ATGP2764-10 µg, ATGP2764-20 µg, ATGP2764-50 µg, ATGP2764-100 µg, ATGP2764-250 µg, ATGP2764-500 µg, ATGP2764-1 mg, ATGP2764-10, ATGP2764-20, ATGP2764-50, ATGP2764-100, ATGP2764-250, ATGP2764-500, ATGP2764-1

• References & Citations:

  Yamashita A., et al (2009) . Genes Dev. 23:1091-1105; ; Olsen J.V., et al (2006) . Cell 127:635-648

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

• Scientific Category:

  Epigenomics (Transcription & Translation)

• NCBI Accession Number:

  NP_001952.1

• Uniprot Accession Number:

  P13639

• Species:

  Human

• AA Sequence:

  DPVVSYR ETVSEESNVL CLSKSPNKHN RLYMKARPFP DGLAEDIDKG EVSARQELKQ RARYLAEKYE WDVAEARKIW CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA LDNFLDKL