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Recombinant human GARS protein

CAT: 0112-ATGP2756-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2756-050Size:50 µg
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Description
GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This enzyme is an (alpha) 2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Recombinant human GARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
30 kDa (270aa) confirmed by MALDI-TOF
Additionnal Information
GARS, Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1, ATGP2756-10 µg, ATGP2756-20 µg, ATGP2756-50 µg, ATGP2756-100 µg, ATGP2756-250 µg, ATGP2756-500 µg, ATGP2756-1 mg, ATGP2756-10, ATGP2756-20, ATGP2756-50, ATGP2756-100, ATGP2756-250, ATGP2756-500, ATGP2756-1
References & Citations
Guo R.-T., et al. (2009) J. Biol. Chem. 284:28968-28976; ; Xie W., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:9976-9981;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_002038
Uniprot Accession Number
P41250
Species
Human
AA Sequence
PISLPAA ASRSSMDGAG AEEVLAPLRL AVRQQGDLVR KLKEDKAPQV DVDKAVAELK ARKRVLEAKE LALQPKDDIV DRAKMEDTLK RRFFYDQAFA IYGGVSGLYD FGPVGCALKN NIIQTWRQHF IQEEQILEID CTMLTPEPVL KTSGHVDKFA DFMVKDVKNG ECFRADHLLK AHLQKLMSDK KCSVEKKSEM ESVLAQLDNY GQQELADLFV NYNVKSPITG NDLSPPVSFN LMFKTFIGPG

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