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Recombinant human RNF34 protein

CAT: 0112-ATGP2419-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2419-500Size:500 µg
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Description
RNF34contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. Recombinant human RNF34 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Name Alternative
E3 ubiquitin-protein ligase RNF34 isoform 1, CARP-1, CARP1, Caspase regulator CARP1, Caspases-8 and-10-associated RING finger protein 1, E3 ubiquitin-protein ligase, ring finger protein 34, FLJ21786, RIF, RIFF, E3 ubiquitin protein ligase RNF34 isoform 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
44.2 kDa (396aa) confirmed by MALDI-TOF
Additionnal Information
RNF34, E3 ubiquitin-protein ligase RNF34 isoform 1, CARP-1, CARP1, Caspase regulator CARP1, Caspases-8 and-10-associated RING finger protein 1, E3 ubiquitin-protein ligase, ring finger protein 34, FLJ21786, RIF, RIFF, E3 ubiquitin protein ligase RNF34 isoform 1, ATGP2419-10 µg, ATGP2419-20 µg, ATGP2419-50 µg, ATGP2419-100 µg, ATGP2419-250 µg, ATGP2419-500 µg, ATGP2419-1 mg, ATGP2419-10, ATGP2419-20, ATGP2419-50, ATGP2419-100, ATGP2419-250, ATGP2419-500, ATGP2419-1
References & Citations
Wei P. et al. (2012) Mol Cell Biol. 32:266-275.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl, 1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_919247
Uniprot Accession Number
Q969K3
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMRKAGAT SMWASCCGLL NEVMGTGAVR GQQSAFAGAT GPFRFTPNPE FSTYPPAATE GPNIVCKACG LSFSVFRKKH VCCDCKKDFC SVCSVLQENL RRCSTCHLLQ ETAFQRPQLM RLKVKDLRQY LILRNIPIDT CREKEDLVDL VLCHHGLGSE DDMDTSSLNS SRSQTSSFFT RSFFSNYTAP SATMSSFQGE LMDGDQTSRS GVPAQVQSEI TSANTEDDDD DDDEDDDDEE ENAEDRNPGL SKERVRASLS DLSSLDDVEG MSVRQLKEIL ARNFVNYSGC CEKWELVEKV NRLYKENEEN QKSYGERLQL QDEEDDSLCR ICMDAVIDCV LLECGHMVTC TKCGKRMSEC PICRQYVVRA VHVFKS

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