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Recombinant human eIF-3 beta/EIF3I protein

CAT: 0112-ATGP2393-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2393-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3I protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Name Alternative
C3orf68, eIF3-beta, eIF-3-beta, eIF3i, eIF3-p36, EIF3S2, Eukaryotic translation initiation factor 3 subunit 2, Eukaryotic translation initiation factor 3 subunit 2 beta 36kDa, Eukaryotic translation initiation factor 3 subunit I, PRO2242, TGF-beta receptor-interacting protein 1, TRIP1, TRIP-1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
38.9 kDa (348aa)
Additionnal Information
EIF3I, eukaryotic translation initiation factor 3 subunit I, C3orf68, ATGP2393-10 µg, ATGP2393-20 µg, ATGP2393-50 µg, ATGP2393-100 µg, ATGP2393-250 µg, ATGP2393-500 µg, ATGP2393-1 mg, ATGP2393-10, ATGP2393-20, ATGP2393-50, ATGP2393-100, ATGP2393-250, ATGP2393-500, ATGP2393-1
References & Citations
Proshkin, S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980; ; Perez, R.E., et al. (2011) Am. J. Physiol. Lung Cell Mol. Physiol. 300 (5), L799-L807;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_003748
Uniprot Accession Number
Q13347
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMKPILLQ GHERSITQIK YNREGDLLFT VAKDPIVNVW YSVNGERLGT YMGHTGAVWC VDADWDTKHV LTGSADNSCR LWDCETGKQL ALLKTNSAVR TCGFDFGGNI IMFSTDKQMG YQCFVSFFDL RDPSQIDNNE PYMKIPCNDS KITSAVWGPL GECIIAGHES GELNQYSAKS GEVLVNVKEH SRQINDIQLS RDMTMFVTAS KDNTAKLFDS TTLEHQKTFR TERPVNSAAL SPNYDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFEFEFEA

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