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Recombinant human DBR1 protein

CAT: 0112-ATGP2340-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2340-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DBR1 is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. Recombinant human DBR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Lariat debranching enzyme, Debranching enzyme homolog 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
38 kDa (340aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
DBR1, Lariat debranching enzyme, Debranching enzyme homolog 1, ATGP2340-10 µg, ATGP2340-20 µg, ATGP2340-50 µg, ATGP2340-100 µg, ATGP2340-250 µg, ATGP2340-500 µg, ATGP2340-1 mg, ATGP2340-10, ATGP2340-20, ATGP2340-50, ATGP2340-100, ATGP2340-250, ATGP2340-500, ATGP2340-1
References & Citations
Ye Y, De Leon J, et al. (2005) . Retrovirology. 2:63.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_057300
Uniprot Accession Number
Q9UK59
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA

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