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Recombinant human PPM1F protein

CAT: 0112-ATGP2281-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2281-100Size:100 µg
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Description
PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
52.2 kDa (477aa)
Additionnal Information
PPM1F, Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2, ATGP2281-10 µg, ATGP2281-20 µg, ATGP2281-50 µg, ATGP2281-100 µg, ATGP2281-250 µg, ATGP2281-500 µg, ATGP2281-1 mg, ATGP2281-10, ATGP2281-20, ATGP2281-50, ATGP2281-100, ATGP2281-250, ATGP2281-500, ATGP2281-1
References & Citations
Tan KM, Chan SL, et al. (2001) . J Biol Chem. 276 (47) :44193-202. ; ; Harvey BP, Banga SS, et al. (2004) . J Biol Chem. 279 (23) :24889-98.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_055449
Uniprot Accession Number
P49593
Species
Human
AA Sequence
MSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE GDPQAEGRRQ DLPSSLPEPE TQAPPRS

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