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Recombinant human Prohibitin protein

CAT: 0112-ATGP1987-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1987-050Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PHB is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. Recombinant human PHB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Prohibitin, PHB1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
31.9 kDa (292aa) confirmed by MALDI-TOF
Additionnal Information
Prohibitin, Prohibitin, PHB1, ATGP1987-10 µg, ATGP1987-20 µg, ATGP1987-50 µg, ATGP1987-100 µg, ATGP1987-250 µg, ATGP1987-500 µg, ATGP1987-1 mg, ATGP1987-10, ATGP1987-20, ATGP1987-50, ATGP1987-100, ATGP1987-250, ATGP1987-500, ATGP1987-1
References & Citations
Van Aken O. et al. (2007) Plant J. 52:850-864.; ; Mishra S. et al. (2006) J Cell Mol Med. 10:353-363.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_002625.1
Uniprot Accession Number
P35232
Species
Human
AA Sequence
MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ LSRSRNITYL PAGQSVLLQL PQ

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