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Recombinant human RNF181 protein

CAT: 0112-ATGP1717-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1717-100Size:100 µg
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Description
E3 ubiquitin-protein ligase RNF181, also known as RNF181, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Recombinant human RNF181 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
E3 ubiquitin-protein ligase RNF181, HSPC238
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
20.3 kDa (176aa) confirmed by MALDI-TOF
Additionnal Information
RNF181, E3 ubiquitin-protein ligase RNF181, HSPC238, ATGP1717-10 µg, ATGP1717-20 µg, ATGP1717-50 µg, ATGP1717-100 µg, ATGP1717-250 µg, ATGP1717-500 µg, ATGP1717-1 mg, ATGP1717-10, ATGP1717-20, ATGP1717-50, ATGP1717-100, ATGP1717-250, ATGP1717-500, ATGP1717-1
References & Citations
Brophy T.M., et al. (2008) Biochem. Biophys. Res. Commun. 369:1088-1093; ; Gauci S., et al. (2009) Anal. Chem. 81:4493-4501
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_057578
Uniprot Accession Number
Q9P0P0
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASYFDE HDCEPSDPEQ ETRTNMLLEL ARSLFNRMDF EDLGLVVDWD HHLPPPAAKT VVENLPRTVI RGSQAELKCP VCLLEFEEEE TAIEMPCHHL FHSSCILPWL SKTNSCPLCR YELPTDDDTY EEHRRDKARK QQQQHRLENL HGAMYT

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