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Recombinant human GCLM protein

CAT: 0112-ATGP1657-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1657-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Recombinant human GCLM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
33.3 kDa (298aa) confirmed by MALDI-TOF
Additionnal Information
GCLM, Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR, ATGP1657-10 µg, ATGP1657-20 µg, ATGP1657-50 µg, ATGP1657-100 µg, ATGP1657-250 µg, ATGP1657-500 µg, ATGP1657-1 mg, ATGP1657-10, ATGP1657-20, ATGP1657-50, ATGP1657-100, ATGP1657-250, ATGP1657-500, ATGP1657-1
References & Citations
Hanzawa, R., et al. (2011) Psychiatry Clin. Neurosci. 65 (1), 39-46
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_002052
Uniprot Accession Number
P48507
Species
Human
AA Sequence
MGTDSR AAKALLARAR TLHLQTGNLL NWGRLRKKCP STHSEELHDC IQKTLNEWSS QINPDLVREF PDVLECTVSH AVEKINPDER EEMKVSAKLF IVESNSSSST RSAVDMACSV LGVAQLDSVI IASPPIEDGV NLSLEHLQPY WEELENLVQS KKIVAIGTSD LDKTQLEQLY QWAQVKPNSN QVNLASCCVM PPDLTAFAKQ FDIQLLTHND PKELLSEASF QEALQESIPD IQAHEWVPLW LLRYSVIVKS RGIIKSKGYI LQAKRRGS

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