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Recombinant human EYA2 protein

CAT: 0112-ATGP1478-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1478-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EYA2, also known as eyes absent homolog 2, is a member of tyrosine phosphatase family that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) . This protein promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Recombinant human EYA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Eyes absent homolog 2, EAB1, MGC10614
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
33.2 kDa (295aa) confirmed by MALDI-TOF
Additionnal Information
EYA2, Eyes absent homolog 2, EAB1, MGC10614, ATGP1478-10 µg, ATGP1478-20 µg, ATGP1478-50 µg, ATGP1478-100 µg, ATGP1478-250 µg, ATGP1478-500 µg, ATGP1478-1 mg, ATGP1478-10, ATGP1478-20, ATGP1478-50, ATGP1478-100, ATGP1478-250, ATGP1478-500, ATGP1478-1
References & Citations
Krishnan N., et al. (2009) J. Biol. Chem. 284:16066-16070; ; Fan X., et al. (2000) J. Biol. Chem. 275:32129-32134
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
AAH08803
Uniprot Accession Number
O00167
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMERVFVW DLDETIIIFH SLLTGTFASR YGKDTTTSVR IGLMMEEMIF NLADTHLFFN DLEDCDQIHV DDVSSDDNGQ DLSTYNFSAD GFHSSAPAAN LCLGSGVHGG VDWMRKLAFR YRRVKEMYNT YKNNVGGLIG TPKRETWLQL RAELEALTDL WLTHSLKALN LINSRPNCVN VLVTTTQLIP ALAKVLLYGL GSVFPIENIY SATKTGKESC FERIMQRFGR KAVYVVIGDG VEEEQGAKKH NMPFWRISCH ADLEALRHAL ELEYL

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