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Recombinant human ATG4B protein

CAT: 0112-ATGP1431-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1431-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II) . Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) -conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Cysteine protease ATG4B, AuTL1, Autophagin-1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
45.3 kDa (401aa) confirmed by MALDI-TOF
Additionnal Information
ATG4B, Cysteine protease ATG4B, AuTL1, Autophagin-1, ATGP1431-10 µg, ATGP1431-20 µg, ATGP1431-50 µg, ATGP1431-100 µg, ATGP1431-250 µg, ATGP1431-500 µg, ATGP1431-1 mg, ATGP1431-10, ATGP1431-20, ATGP1431-50, ATGP1431-100, ATGP1431-250, ATGP1431-500, ATGP1431-1
References & Citations
Sugawara K., et al. (2005) J. Biol. Chem. 280:40058-40065; ; Kumanomidou T., et al. (2006) J. Mol. Biol. 355:612-618;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF
Scientific Category
Ubiquitination
NCBI Accession Number
AAH00719.1
Uniprot Accession Number
Q9Y4P1
Species
Human

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