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Recombinant human ASPA protein

CAT: 0112-ATGP1421-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1421-100Size:100 µg
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Description
ASPA, also known as aspartoacylase, is expressed in liver, lung and kidney tissue, as well as in skeletal muscle and in cerebral white matter. Existing as a homodimer, Aspartoacylase functions to catalyze the deacetylation of N-acetylaspartic acid (NAA) (a protein whose hydrolysis is crucial to maintenance of intact white matter) to produce acetate and L-aspartate. Recombinant human ASPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Aspartoacylase, ACY2, ASP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
38.1 kDa (336aa) confirmed by MALDI-TOF
Additionnal Information
ASPA, Aspartoacylase, ACY2, ASP, ATGP1421-10 µg, ATGP1421-20 µg, ATGP1421-50 µg, ATGP1421-100 µg, ATGP1421-250 µg, ATGP1421-500 µg, ATGP1421-1 mg, ATGP1421-10, ATGP1421-20, ATGP1421-50, ATGP1421-100, ATGP1421-250, ATGP1421-500, ATGP1421-1
References & Citations
Le Coq. et al. (2006) Biochemistry 45: 5878-5884.; ; Le Coq. et al. (2008) Biochemistry 47: 3484-3492.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSF
Scientific Category
Neuroscience
NCBI Accession Number
NP_000040.1
Uniprot Accession Number
P45381
Species
Human
AA Sequence
MTSCHIA EEHIQKVAIF GGTHGNELTG VFLVKHWLEN GAEIQRTGLE VKPFITNPRA VKKCTRYIDC DLNRIFDLEN LGKKMSEDLP YEVRRAQEIN HLFGPKDSED SYDIIFDLHN TTSNMGCTLI LEDSRNNFLI QMFHYIKTSL APLPCYVYLI EHPSLKYATT RSIAKYPVGI EVGPQPQGVL RADILDQMRK MIKHALDFIH HFNEGKEFPP CAIEVYKIIE KVDYPRDENG EIAAIIHPNL QDQDWKPLHP GDPMFLTLDG KTIPLGGDCT VYPVFVNEAA YYEKKEAFAK TTKLTLNAKS IRCCLH

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