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Recombinant e.coli coaA protein

CAT: 0112-ATGP1395-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1395-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CoaA (pantothenate kinase) belongs to the prokaryotic pantothenate kinase family. Pantothenate kinase (PanK; CoaA) is the first enzyme in the Coenzyme A biosynthetic pathway. It phophorylates pantothenate (vitamin B5) to form 4'-phosphopantothenate. Regulation of pantothenate kinase activity by feedback inhibition is the critical factor controlling the intracellular CoA concentration. Recombinant E. coli coaA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Pantothenate kinase, panK, rts, ts-9
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
38.9 kDa (340aa) confirmed by MALDI-TOF
Additionnal Information
CoaA, pantothenate kinase, panK, rts, ts-9, ATGP1395-10 µg, ATGP1395-20 µg, ATGP1395-50 µg, ATGP1395-100 µg, ATGP1395-250 µg, ATGP1395-500 µg, ATGP1395-1 mg, ATGP1395-10, ATGP1395-20, ATGP1395-50, ATGP1395-100, ATGP1395-250, ATGP1395-500, ATGP1395-1
References & Citations
Song W.-J., et al. (1992) J.Bacteriol. 174:6411-6417; ; Song W.-J., et al. (1992) J.Bacteriol. 174:1705-1706
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_418405
Uniprot Accession Number
P0A6I3
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSIKEQ TLMTPYLQFD RNQWAALRDS VPMTLSEDEI ARLKGINEDL SLEEVAEIYL PLSRLLNFYI SSNLRRQAVL EQFLGTNGQR IPYIISIAGS VAVGKSTTAR VLQALLSRWP EHRRVELITT DGFLHPNQVL KERGLMKKKG FPESYDMHRL VKFVSDLKSG VPNVTAPVYS HLIYDVIPDG DKTVVQPDIL ILEGLNVLQS GMDYPHDPHH VFVSDFVDFS IYVDAPEDLL QTWYINRFLK FREGAFTDPD SYFHNYAKLT KEEAIKTAMT LWKEINWLNL KQNILPTRER ASLILTKSAN HAVEEVRLRK

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