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Recombinant human ATF1 protein

CAT: 0112-ATGP1374-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1374-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Activating transcription factor 1, also known as ATF1, is a cyclic-AMP dependent transcription factor. ATF1 is expressed in a wide variety of cell types and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinases are required for the stress-induced phosphorylation of transcription factors CREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factor induction of c-jun expression requires ATF1 and MEF2 sites in the c-jun promoter. Recombinant human ATF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Activating transcription factor 1, EWS-ATF1, FuS/ATF-1, TREB36
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
31.8 kDa (295aa) confirmed by MALDI-TOF
Additionnal Information
ATF1, Activating transcription factor 1, EWS-ATF1, FuS/ATF-1, TREB36, ATGP1374-10 µg, ATGP1374-20 µg, ATGP1374-50 µg, ATGP1374-100 µg, ATGP1374-250 µg, ATGP1374-500 µg, ATGP1374-1 mg, ATGP1374-10, ATGP1374-20, ATGP1374-50, ATGP1374-100, ATGP1374-250, ATGP1374-500, ATGP1374-1
References & Citations
Lin Y S., et al. (1988) Proc Natl Acad Sci uSA. 85:3396-3400.; ; Gupta P., et al. (2002) J Biol Chem. 277:50550-50556.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005162.1
Uniprot Accession Number
P18846
Species
Human
AA Sequence
MEDSHK STTSETAPQP GSAVQGAHIS HIAQQVSSLS ESEESQDSSD SIGSSQKAHG ILARRPSYRK ILKDLSSEDT RGRKGDGENS GVSAAVTSMS VPTPIYQTSS GQYIAIAPNG ALQLASPGTD GVQGLQTLTM TNSGSTQQGT TILQYAQTSD GQQILVPSNQ VVVQTASGDM QTYQIRTTPS ATSLPQTVVM TSPVTLTSQT TKTDDPQLKR EIRLMKNREA ARECRRKKKE YVKCLENRVA VLENQNKTLI EELKTLKDLY SNKSV

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