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Recombinant human CDK2 protein

CAT: 0112-ATGP1180-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1180-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CDK2, also known as cyclin-depentent kinase 2, is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) . Its activity is also regulated by protein phosphorylation. Recombinant human CDK2 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Cyclin-depentent kinase 2, p33, CDK2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
35.0 kDa (306aa) confirmed by MALDI-TOF
Additionnal Information
CDK2, Cyclin-depentent kinase 2, p33, CDK2, ATGP1180-10 µg, ATGP1180-20 µg, ATGP1180-50 µg, ATGP1180-100 µg, ATGP1180-250 µg, ATGP1180-500 µg, ATGP1180-1 mg, ATGP1180-10, ATGP1180-20, ATGP1180-50, ATGP1180-100, ATGP1180-250, ATGP1180-500, ATGP1180-1
References & Citations
Gyuris J., et al. (1993) Cell. 75:791-803; ; Hannon G.J., et al. (1994) Proc. Natl. Acad. Sci. u.S.A. 91:1731-1735
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_001789.2
Uniprot Accession Number
P24941
Species
Human

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