Recombinant human KARS protein

• Description :

  KARS, also known as Lysyl-tRNA synthetase, belongs to the class-II aminoacyl-tRNA synthetase family. It exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. This protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Recombinant human KARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

• Product Name Alternative :

  Lysyl-tRNA synthetase, CMTRIB, KARS2, KIAA0070, KRS

• Expression System :

  E.coli

• Antigen Species :

  Human

• Tag :

  His-Tag

• Applications :

  SDS-PAGE

• Concentration :

  1 mg/mL (determined by Bradford assay)

• Purity :

  > 95% by SDS-PAGE

• Molecular Weight :

  63.7 kDa (558aa)

• Additionnal Information :

  KARS, Lysyl-tRNA synthetase, CMTRIB, KARS2, KIAA0070, KRS, ATGP1166-10 µg, ATGP1166-20 µg, ATGP1166-50 µg, ATGP1166-100 µg, ATGP1166-250 µg, ATGP1166-500 µg, ATGP1166-1 mg, ATGP1166-10, ATGP1166-20, ATGP1166-50, ATGP1166-100, ATGP1166-250, ATGP1166-500, ATGP1166-1

• References & Citations :

  Targoff I.N. et al. (1993) J. Clin. Invest. 91: 2556-2564.; ; Tolkunova E. et al. (2000) J. Biol. Chem. 275: 35063-35069.

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

• Scientific Category :

  Enzymes & Proteases

• NCBI Accession Number :

  NP_005539.1

• Uniprot Accession Number :

  Q15046

• Species :

  Human

• AA Sequence :

  GVGPEEE SVDPNQYYKI RSQAIHQLKV NGEDPYPHKF HVDISLTDFI QKYSHLQPGD HLTDITLKVA GRIHAKRASG GKLIFYDLRG EGVKLQVMAN SRNYKSEEEF IHINNKLRRG DIIGVQGNPG KTKKGELSII PYEITLLSPC LHMLPHLHFG LKDKETRYRQ RYLDLILNDF VRQKFIIRSK IITYIRSFLD ELGFLEIETP MMNIIPGGAV AKPFITYHNE LDMNLYMRIA PELYHKMLVV GGIDRVYEIG RQFRNEGIDL THNPEFTTCE FYMAYADYHD LMEITEKMVS GMVKHITGSY KVTYHPDGPE GQAYDVDFTP PFRRINMVEE LEKALGMKLP ETNLFETEET RKILDDICVA KAVECPPPRT TARLLDKLVG EFLEVTCINP TFICDHPQIM SPLAKWHRSK EGLTERFELF VMKKEICNAY TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV