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Recombinant human DCPS protein

CAT: 0112-ATGP1162-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1162-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DCPS, also known as scavenger mRNA-decapping enzyme, is belongs to the HIT family. It is necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. A scavenger decapping enzyme, DcpS was shown to hydrolyze the residual m7GpppN cap structure after the complete 3'-5' degradation of the mRNA by the exosome. DcpS releases m7GMP and is unable to cleave cap structures attached to a long RNA chain. Recombinant human DCPS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Scavenger mRNA-decapping enzyme DcpS, DCS1, HINT5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
40.7 kDa (357aa) confirmed by MALDI-TOF
Additionnal Information
DCPS, Scavenger mRNA-decapping enzyme DcpS, DCS1, HINT5, ATGP1162-10 µg, ATGP1162-20 µg, ATGP1162-50 µg, ATGP1162-100 µg, ATGP1162-250 µg, ATGP1162-500 µg, ATGP1162-1 mg, ATGP1162-10, ATGP1162-20, ATGP1162-50, ATGP1162-100, ATGP1162-250, ATGP1162-500, ATGP1162-1
References & Citations
Liu H., et al. (2002) EMBO J. 21:4699-4708; ; Kwasnicka D.A., et al. (2003) J. Biol. Chem. 278:39051-39058
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_054745
Uniprot Accession Number
Q96C86
Species
Human
AA Sequence
MADAAPQLGK RKRELDVEEA HAASTEEKEA GVGNGTCAPV RLPFSGFRLQ KVLRESARDK IIFLHGKVNE ASGDGDGEDA VVILEKTPFQ VEQVAQLLTG SPELQLQFSN DIYSTYHLFP PRQLNDVKTT VVYPATEKHL QKYLRQDLRL IRETGDDYRN ITLPHLESQS LSIQWVYNIL DKKAEADRIV FENPDPSDGF VLIPDLKWNQ QQLDDLYLIA ICHRRGIRSL RDLTPEHLPL LRNILHQGQE AILQRYRMKG DHLRVYLHYL PSYYHLHVHF TALGFEAPGS GVERAHLLAE VIENLECDPR HYQQRTLTFA LRADDPLLKL LQEAQQS

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