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Recombinant human YARS protein

CAT: 0112-ATGP1055-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1055-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tyrosyl-tRNA synthetase, also known as YARS, belongs to the class I tRNA synthetase family. YARS is essential enzyme that catalyze the aminoacylation of tRNATrp with tryptophan, an essential function of the cell's protein synthesis machinery. Expression of YARS is highly stimulated in human cells by the addition of IFN-gamma. Recombinant human YARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Tyrosyl-tRNA synthetase, CMTDIC, TYRRS, YRS, YTS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
61.3 kDa (548aa)
Additionnal Information
YARS, Tyrosyl-tRNA synthetase, CMTDIC, TYRRS, YRS, YTS, ATGP1055-10 µg, ATGP1055-20 µg, ATGP1055-50 µg, ATGP1055-100 µg, ATGP1055-250 µg, ATGP1055-500 µg, ATGP1055-1 mg, ATGP1055-10, ATGP1055-20, ATGP1055-50, ATGP1055-100, ATGP1055-250, ATGP1055-500, ATGP1055-1
References & Citations
Kise Y., et al. (2004) Nat Struct Mol Biol. 11:149-156.; ; Wakasugi K., et al. (2002) Proc Natl Acad Sci . 99:173-177.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_003671.1
Uniprot Accession Number
P54577
Species
Human
AA Sequence
MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPM AKGPAKNSEP EEVIPSRLDI RVGKIITVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVESQGML LCASIEGINR QVEPLDPPAG SAPGEHVFVK GYEKGQPDEE LKPKKKVFEK LQADFKISEE CIAQWKQTNF MTKLGSISCK SLKGGNIS

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