Recombinant human Rap2A protein

• Description:

  RAP2A, also known as RAP2, is a member of RAS oncogene family and a Ras-like small G protein. This protein shares 60% identity with Rap1A and exhibits a carboxy-terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap1A and Rap1B, overexpression of RAP2A does not interfere with the Ras signaling pathway. Recombinant human RAP2A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative:

  Ras-related protein Rap-2a, K-REV, KREV, RAP2, RbBP-30

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Purity:

  > 95% by SDS-PAGE

• Molecular Weight:

  22.4 kDa (200aa) confirmed by MALDI-TOF

• Additionnal Information:

  Rap2A, Ras-related protein Rap-2a, K-REV, KREV, RAP2, RbBP-30, ATGP0855-10 µg, ATGP0855-20 µg, ATGP0855-50 µg, ATGP0855-100 µg, ATGP0855-250 µg, ATGP0855-500 µg, ATGP0855-1 mg, ATGP0855-10, ATGP0855-20, ATGP0855-50, ATGP0855-100, ATGP0855-250, ATGP0855-500, ATGP0855-1

• References & Citations:

  Yaman E., et al. (2009) FEBS J. 276 (16) :4607-16.; ; Das R., et al. (2008) J Biol Chem. 283 (28) :19691-703.;

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

• Scientific Category:

  Signal Transduction

• NCBI Accession Number:

  NP_066361

• Uniprot Accession Number:

  P10114

• Species:

  Human

• AA Sequence:

  MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI IRVKRYEKVP VILVGNKVDL ESEREVSSSE GRALAEEWGC PFMETSAKSK TMVDELFAEI VRQMNYAAQP DKDDPCCSAC