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Recombinant human MAGEA3 protein

CAT: 0112-ATGP0757-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0757-250Size:250 µg
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Description
Melanoma-associated antigen 3, also known MAGEA3, is a member of the MAGE gene family, which comprises 12 known genes, of which 6 are expressed in tumors. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGEA3 is a tumor-specific antigen widely expressed in solid and hematologic malignancies, but not in normal tissues, with the exception of testis and placenta. Therefore, MAGEA3 is an excellent candidate tumor antigen. Recombinant human MAGEA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Melanoma-associated antigen 3, CT1.3, HIP8, HYPD, MAGE3, MZ2 D
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
37.1 kDa (337aa) confirmed by MALDI-TOF
Additionnal Information
MAGEA3, Melanoma-associated antigen 3, CT1.3, HIP8, HYPD, MAGE3, MZ2 D, ATGP0757-10 µg, ATGP0757-20 µg, ATGP0757-50 µg, ATGP0757-100 µg, ATGP0757-250 µg, ATGP0757-500 µg, ATGP0757-1 mg, ATGP0757-10, ATGP0757-20, ATGP0757-50, ATGP0757-100, ATGP0757-250, ATGP0757-500, ATGP0757-1
References & Citations
Roqner u C., et al. (1995) Genomics. 29 (3) :725-31.; ; Jang S J., et al. (2001) Cancer Res. 61 (21) :7959-63.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Cancer
NCBI Accession Number
NP_005353.1
Uniprot Accession Number
P43357
Species
Human
AA Sequence
PLEQRS QHCKPEEGLE ARGEALGLVG AQAPATEEQE AASSSSTLVE VTLGEVPAAE SPDPPQSPQG ASSLPTTMNY PLWSQSYEDS SNQEEEGPST FPDLESEFQA ALSRKVAELV HFLLLKYRAR EPVTKAEMLG SVVGNWQYFF PVIFSKASSS LQLVFGIELM EVDPIGHLYI FATCLGLSYD GLLGDNQIMP KAGLLIIVLA IIAREGDCAP EEKIWEELSV LEVFEGREDS ILGDPKKLLT QHFVQENYLE YRQVPGSDPA CYEFLWGPRA LVETSYVKVL HHMVKISGGP HISYPPLHEW VLREGEE

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