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Recombinant human ERK1/MAPK3 protein

CAT: 0112-ATGP0687-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0687-500Size:500 µg
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Description
MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This protein is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Recombinant human MAPK3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, PRKM3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
45.2 kDa (399aa) confirmed by MALDI-TOF
Additionnal Information
PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, p44 MAPK, p44 ERK1, Mitogen-activated protein kinase 3, Mitogen activated protein kinase 3, Microtubule-associated protein 2 kinase, Microtubule associated protein 2 kinase, MAPK-3, MAPK3, MAPK 3, MAP kinase3, MAP kinase isoform p44, MAP kinase 3, Insulin-stimulated MAP2 kinase, Insulin stimulated MAP2 kinase, HuMKER1A, HS44KDAP, Extracellular signal-regulated kinase 1, Extracellular signal regulated kinase 1, ERT-2, ERT2, ERT 2, ERK-2, ERK2, ERK-1, ERK1, ERK 2, ERK 1, ATGP0687-10 µg, ATGP0687-20 µg, ATGP0687-50 µg, ATGP0687-100 µg, ATGP0687-250 µg, ATGP0687-500 µg, ATGP0687-1 mg, ATGP0687-010, ATGP0687-020, ATGP0687-050, ATGP0687-100, ATGP0687-250, ATGP0687-500, ATGP0687-01M
References & Citations
Kim DW, et al. (2000) Mol Cell Biol. 20 (4) :1140-8
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Cell Cycle
NCBI Accession Number
NP_002737
Uniprot Accession Number
P27361
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS NRPIFPGKHY LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD SKALDLLDRM LTFNPNKRIT VEEALAHPYL EQYYDPTDEP VAEEPFTFAM ELDDLPKERL KELIFQETAR FQPGVLEAP

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